chem impex international inc  (Chem Impex International)

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Schematic workflow and timeline for cultivation and antimicrobial susceptibility testing of M. hyorhinis . ( A ) According to CLSI, at least two subcultures of a properly grown culture are needed. Growth is characterized by a color change of the medium (pH shift) from red (no growth/growth control—left tube) to yellow (grown culture—right tube). ( B ) Cultures are serially diluted (tenfold) ( B1 ) over five steps; 20 µL of each dilution step, as well as the original culture, are dropped onto a Friis agar plate ( B2 ). The agar plate was divided into six sections. The area of the inoculum was encircled so that the area of growth can later be found easily. After incubation for up to 14 days, each section was evaluated for the presence of colonies using a stereo microscope. ( B3 ) Sections with 30 to 300 single colonies were used for calculating the CFU/mL ( C ) Depicted is an example of a microtiter plate after the maximum time of incubation (14 days), inoculated with the type strain M. hyorhinis DSM 25591.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques: Control, Incubation, Microscopy

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Color of inoculum in wells of a microtiter plate after 14 days of incubation. All plates were incubated at 37 °C and ambient air. ( A ) modified Friis broth without mycoplasmas ( B ) M. hyorhinis DSM 25591, no visible growth and no color change (red). ( C ) M. hyorhinis DSM 25591, visible growth indicated by complete color change (yellow). ( D ) M. hyorhinis DSM 25591, trailing with incomplete color change (orange).

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques: Incubation, Modification

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Growth curves of ( a ) M. hyorhinis DSM 25591 and ( b ) M. hyopneumoniae NCTC 10110 cultured in modified Friis broth (black) or SP4 broth (red). Both strains were cultured in triplicates. The cell count was determined on the corresponding agar media.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques: Cell Culture, Modification, Cell Counting

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Growth curves of seven M. hyorhinis field isolates cultured in modified Friis broth for eleven days.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques: Cell Culture, Modification

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Distribution of the MIC values of M. hyorhinis DSM 25591 obtained in 22 individual tests.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques:

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Homogeneity of the MIC values of the 22 test replicates of M. hyorhinis DSM 25591, as given in Table 2 . The grey shaded area describes the MIC values accounting for the essential MIC agreement.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques:

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Distribution of the MIC values for M. hyorhinis DSM 25591. Twenty-one individual test results containing all tested batches and the respective controls are shown. For reasons of better comparability, the initial ranges of MIC results, given in Table 2 , are highlighted in green.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques:

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Homogeneity of the MIC values of 21 test replicates of M. hyorhinis DSM 25591 tested in modified Friis broth, prepared with different batches of ingredients. The mode MIC and its deviations were determined based on the results of the initial testing of M. hyorhinis DSM 25591 (see Table 2 ). The grey shaded area contains the MIC values accounting for the essential MIC agreement.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques: Modification

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Distribution of the MIC values of six M. hyorhinis field isolates using the modified Friis broth. The MIC values represent the mode MICs of three independent tests of each isolate.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques: Modification

Journal: Microorganisms

Article Title: Towards a Standardized Antimicrobial Susceptibility Testing Method for Mycoplasma hyorhinis

doi: 10.3390/microorganisms11040994

Figure Lengend Snippet: Homogeneity of the MIC values of six M. hyorhinis field isolates tested in modified Friis broth, prepared with different batches of ingredients. The mode MIC and its deviations were determined, based on the results of the initial testing of the isolates (see Table 6 ). The grey-shaded area contains the MIC values accounting for the essential MIC agreement.

Article Snippet: In agreement with the published literature, the type strain of M. hyorhinis (e.g., DSM 25591, ATCC 17981, NCTC 10130) provided repeatable results and, thus, might be proposed as a future quality control strain.

Techniques: Modification